Toward a Systematic Understanding of mRNA 3' Untranslated Regions
نویسندگان
چکیده
منابع مشابه
Regulatory 3′ Untranslated Regions of Bacterial mRNAs
The untranslated regions (UTRs) of mRNA contain important features that are relevant to the post-transcriptional and translational regulation of gene expression. Most studies of bacterial UTRs have focused on the 5'regions; however, 3'UTRs have recently emerged as a new class of post-transcriptional regulatory elements. 3'UTRs were found to regulate the decay and translation initiation in their...
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BACKGROUND Deliberate cellular reprogramming is becoming a realistic objective in the clinic. While the origin of the target cells is critical, delivery of bioactive molecules to trigger a shift in cell-fate remains the major hurdle. To date, several strategies based either on non-integrative vectors, protein transfer or mRNA delivery have been investigated. In a recent study, a unique modifica...
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Proteins that regulate mRNA metabolism are often bipartite: an RNA binding activity confers substrate specificity, and a "functional" domain elicits the biological outcome. In some cases, these two activities reside on different polypeptides that form a complex on the mRNA. Regardless, experimental separation of RNA binding from function facilitates analysis of both properties, liberating each ...
متن کاملDom34 Rescues Ribosomes in 3′ Untranslated Regions
Ribosomes that stall before completing peptide synthesis must be recycled and returned to the cytoplasmic pool. The protein Dom34 and cofactors Hbs1 and Rli1 can dissociate stalled ribosomes in vitro, but the identity of targets in the cell is unknown. Here, we extend ribosome profiling methodology to reveal a high-resolution molecular characterization of Dom34 function in vivo. Dom34 removes s...
متن کاملSystematic genomic screening and analysis of mRNA in untranslated regions and mRNA precursors: combining experimental and computational approaches
MOTIVATION The untranslated regions (UTRs) of mRNA upstream (5'UTR) and downstream (3'UTR) of the open reading frame, as well as the mRNA precursor, carry important regulatory sequences. To reveal unidentified regulatory signals, we combine information from experiments with computational approaches. Depending on available knowledge, three different strategies are employed. RESULTS Searching w...
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ژورنال
عنوان ژورنال: Proceedings of the American Thoracic Society
سال: 2011
ISSN: 1546-3222
DOI: 10.1513/pats.201007-054ms